diff --git a/programs/ssl/ssl_test_lib.c b/programs/ssl/ssl_test_lib.c
index 6e0c6153f..b49dd67c2 100644
--- a/programs/ssl/ssl_test_lib.c
+++ b/programs/ssl/ssl_test_lib.c
@@ -435,6 +435,9 @@ int test_hooks_failure_detected(void)
 
 void test_hooks_free(void)
 {
+#if defined(MBEDTLS_TEST_MUTEX_USAGE)
+    mbedtls_test_mutex_usage_end();
+#endif
 }
 
 #endif /* MBEDTLS_TEST_HOOKS */
diff --git a/tests/include/test/helpers.h b/tests/include/test/helpers.h
index ba117fbdf..4708df163 100644
--- a/tests/include/test/helpers.h
+++ b/tests/include/test/helpers.h
@@ -240,10 +240,18 @@ int mbedtls_test_hexcmp(uint8_t *a, uint8_t *b,
 #endif
 
 #if defined(MBEDTLS_TEST_MUTEX_USAGE)
-/** Permanently activate the mutex usage verification framework. See
- * threading_helpers.c for information. */
+/**
+ *  Activate the mutex usage verification framework. See threading_helpers.c for
+ *  information.
+ *  */
 void mbedtls_test_mutex_usage_init(void);
 
+/**
+ *  Deactivate the mutex usage verification framework. See threading_helpers.c
+ *  for information.
+ */
+void mbedtls_test_mutex_usage_end(void);
+
 /** Call this function after executing a test case to check for mutex usage
  * errors. */
 void mbedtls_test_mutex_usage_check(void);
diff --git a/tests/src/threading_helpers.c b/tests/src/threading_helpers.c
index 385a07926..434d124f1 100644
--- a/tests/src/threading_helpers.c
+++ b/tests/src/threading_helpers.c
@@ -228,4 +228,14 @@ void mbedtls_test_mutex_usage_check(void)
     mbedtls_test_info.mutex_usage_error = NULL;
 }
 
+void mbedtls_test_mutex_usage_end(void)
+{
+    mbedtls_mutex_init = mutex_functions.init;
+    mbedtls_mutex_free = mutex_functions.free;
+    mbedtls_mutex_lock = mutex_functions.lock;
+    mbedtls_mutex_unlock = mutex_functions.unlock;
+
+    mutex_functions.free(&mbedtls_test_mutex_mutex);
+}
+
 #endif /* MBEDTLS_TEST_MUTEX_USAGE */
diff --git a/tests/suites/host_test.function b/tests/suites/host_test.function
index d8ff49ef1..cc286973c 100644
--- a/tests/suites/host_test.function
+++ b/tests/suites/host_test.function
@@ -772,6 +772,10 @@ int execute_tests(int argc, const char **argv)
     mbedtls_fprintf(stdout, " (%u / %u tests (%u skipped))\n",
                     total_tests - total_errors, total_tests, total_skipped);
 
+#if defined(MBEDTLS_TEST_MUTEX_USAGE)
+    mbedtls_test_mutex_usage_end();
+#endif
+
 #if defined(MBEDTLS_MEMORY_BUFFER_ALLOC_C) && \
     !defined(TEST_SUITE_MEMORY_BUFFER_ALLOC)
 #if defined(MBEDTLS_MEMORY_DEBUG)