diff --git a/nixos/release-combined.nix b/nixos/release-combined.nix
index ffa087bb6f28..9e2109d88b5f 100644
--- a/nixos/release-combined.nix
+++ b/nixos/release-combined.nix
@@ -68,6 +68,7 @@ in rec {
         nixos.tests.chromium.x86_64-linux or []
         (all nixos.tests.firefox)
         (all nixos.tests.firewall)
+        (all nixos.tests.fontconfig-default-fonts)
         (all nixos.tests.gnome3-xorg)
         (all nixos.tests.gnome3)
         (all nixos.tests.pantheon)
diff --git a/nixos/tests/all-tests.nix b/nixos/tests/all-tests.nix
index 557ee78df7c6..14dca7409c46 100644
--- a/nixos/tests/all-tests.nix
+++ b/nixos/tests/all-tests.nix
@@ -87,6 +87,7 @@ in
   flatpak = handleTest ./flatpak.nix {};
   flatpak-builder = handleTest ./flatpak-builder.nix {};
   fluentd = handleTest ./fluentd.nix {};
+  fontconfig-default-fonts = handleTest ./fontconfig-default-fonts.nix {};
   fsck = handleTest ./fsck.nix {};
   fwupd = handleTestOn ["x86_64-linux"] ./fwupd.nix {}; # libsmbios is unsupported on aarch64
   gdk-pixbuf = handleTest ./gdk-pixbuf.nix {};
diff --git a/nixos/tests/fontconfig-default-fonts.nix b/nixos/tests/fontconfig-default-fonts.nix
new file mode 100644
index 000000000000..1991cec92189
--- /dev/null
+++ b/nixos/tests/fontconfig-default-fonts.nix
@@ -0,0 +1,28 @@
+import ./make-test.nix ({ lib, ... }:
+{
+  name = "fontconfig-default-fonts";
+
+  machine = { config, pkgs, ... }: {
+    fonts.enableDefaultFonts = true; # Background fonts
+    fonts.fonts = with pkgs; [
+      noto-fonts-emoji
+      cantarell-fonts
+      twitter-color-emoji
+      source-code-pro
+      gentium
+    ];
+    fonts.fontconfig.defaultFonts = {
+      serif = [ "Gentium Plus" ];
+      sansSerif = [ "Cantarell" ];
+      monospace = [ "Source Code Pro" ];
+      emoji = [ "Twitter Color Emoji" ];
+    };
+  };
+
+  testScript = ''
+    $machine->succeed("fc-match serif | grep '\"Gentium Plus\"'");
+    $machine->succeed("fc-match sans-serif | grep '\"Cantarell\"'");
+    $machine->succeed("fc-match monospace | grep '\"Source Code Pro\"'");
+    $machine->succeed("fc-match emoji | grep '\"Twitter Color Emoji\"'");
+  '';
+})