diff --git a/pkgs/applications/networking/maestral-qt/default.nix b/pkgs/applications/networking/maestral-qt/default.nix
index 9b87073f3f41..2e6ab8bd01f6 100644
--- a/pkgs/applications/networking/maestral-qt/default.nix
+++ b/pkgs/applications/networking/maestral-qt/default.nix
@@ -5,21 +5,41 @@
 , nixosTests
 }:
 
-python3.pkgs.buildPythonApplication rec {
+let
+  inherit (pypkgs) makePythonPath;
+
+  pypkgs = (python3.override {
+    packageOverrides = self: super: {
+      # Use last available version of maestral that still supports PyQt5
+      # Remove this override when PyQt6 is available
+      maestral = super.maestral.overridePythonAttrs (old: rec {
+        version = "1.5.3";
+        src = fetchFromGitHub {
+          owner = "SamSchott";
+          repo = "maestral";
+          rev = "refs/tags/v${version}";
+          hash = "sha256-Uo3vcYez2qSq162SSKjoCkwygwR5awzDceIq8/h3dao=";
+        };
+      });
+    };
+  }).pkgs;
+
+in
+pypkgs.buildPythonApplication rec {
   pname = "maestral-qt";
   version = "1.5.3";
-  disabled = python3.pkgs.pythonOlder "3.6";
+  disabled = pypkgs.pythonOlder "3.6";
 
   src = fetchFromGitHub {
     owner = "SamSchott";
     repo = "maestral-qt";
-    rev = "v${version}";
+    rev = "refs/tags/v${version}";
     sha256 = "sha256-zaG9Zwz9S/SVb7xDa7eXkjLNt1BhA1cQ3I18rVt+8uQ=";
   };
 
   format = "pyproject";
 
-  propagatedBuildInputs = with python3.pkgs; [
+  propagatedBuildInputs = with pypkgs; [
     click
     markdown2
     maestral
@@ -36,8 +56,8 @@ python3.pkgs.buildPythonApplication rec {
     "\${qtWrapperArgs[@]}"
 
     # Add the installed directories to the python path so the daemon can find them
-    "--prefix" "PYTHONPATH" ":" "${lib.concatStringsSep ":" (map (p: p + "/lib/${python3.libPrefix}/site-packages") (python3.pkgs.requiredPythonModules python3.pkgs.maestral.propagatedBuildInputs))}"
-    "--prefix" "PYTHONPATH" ":" "${python3.pkgs.maestral}/lib/${python3.libPrefix}/site-packages"
+    "--prefix PYTHONPATH : ${makePythonPath (pypkgs.requiredPythonModules pypkgs.maestral.propagatedBuildInputs)}"
+    "--prefix PYTHONPATH : ${makePythonPath [ pypkgs.maestral ]}"
   ];
 
   # no tests