diff --git a/pkgs/misc/emulators/py65/default.nix b/pkgs/misc/emulators/py65/default.nix
new file mode 100644
index 000000000000..87439f7dcf91
--- /dev/null
+++ b/pkgs/misc/emulators/py65/default.nix
@@ -0,0 +1,26 @@
+{ lib, fetchPypi, buildPythonApplication }:
+
+buildPythonApplication rec {
+  pname = "py65";
+  version = "1.1.0";
+  format = "wheel";
+
+  src = fetchPypi {
+    inherit pname version format;
+    sha256 = "Q7rjiHJ/Ew985vut/8fVAf/wWYW5aBPSvNPm8A6g1zg=";
+  };
+
+  meta = with lib; {
+    homepage = "https://py65.readthedocs.io/";
+    description = "Emulate 6502-based microcomputer systems in Python";
+    longDescription = ''
+      Py65 includes a program called Py65Mon that functions as a machine
+      language monitor. This kind of program is sometimes also called a
+      debugger. Py65Mon provides a command line with many convenient commands
+      for interacting with the simulated 6502-based system.
+    '';
+    license = licenses.bsd3;
+    maintainers = with maintainers; [ AndersonTorres ];
+    platforms = with platforms; all;
+  };
+}
diff --git a/pkgs/top-level/all-packages.nix b/pkgs/top-level/all-packages.nix
index 2358f862f87f..7b29d1508b0a 100644
--- a/pkgs/top-level/all-packages.nix
+++ b/pkgs/top-level/all-packages.nix
@@ -1743,6 +1743,8 @@ in
 
   bsod = callPackage ../misc/emulators/bsod { };
 
+  py65 = python3Packages.callPackage ../misc/emulators/py65 { };
+
   simh = callPackage ../misc/emulators/simh { };
 
   btrfs-progs = callPackage ../tools/filesystems/btrfs-progs { };