diff --git a/pkgs/applications/science/biology/mrtrix/default.nix b/pkgs/applications/science/biology/mrtrix/default.nix
new file mode 100644
index 000000000000..6959b2caab8a
--- /dev/null
+++ b/pkgs/applications/science/biology/mrtrix/default.nix
@@ -0,0 +1,77 @@
+{ stdenv, lib, fetchFromGitHub, python, makeWrapper
+, eigen, fftw, libtiff, zlib, ants, bc
+, qt5, libGL, libGLU, libX11, libXext
+, withGui ? true }:
+
+stdenv.mkDerivation rec {
+  pname = "mrtrix";
+  version = "3.0_RC3_latest";
+
+  src = fetchFromGitHub {
+    owner  = "MRtrix3";
+    repo   = "mrtrix3";
+    rev    = version;
+    sha256 = "184nv524p8j94qicjy9l288bqcgl2yxqqs55a7042i0gfsnwp51c";
+    fetchSubmodules = true;
+  };
+
+  nativeBuildInputs = [ eigen makeWrapper ] ++ lib.optional withGui qt5.wrapQtAppsHook;
+
+  buildInputs = [
+    ants
+    python
+    fftw
+    libtiff
+    zlib ] ++ lib.optionals withGui [
+    libGL
+    libGLU
+    libX11
+    libXext
+    qt5.qtbase
+    qt5.qtsvg
+  ];
+
+  installCheckInputs = [ bc ];
+
+  postPatch = ''
+    patchShebangs ./build ./configure ./run_tests ./bin/population_template
+    substituteInPlace ./run_tests  \
+      --replace 'git submodule update --init >> $LOGFILE 2>&1' ""
+  '';
+
+  configurePhase = ''
+    export EIGEN_CFLAGS="-isystem ${eigen}/include/eigen3"
+    unset LD  # similar to https://github.com/MRtrix3/mrtrix3/issues/1519
+    ./configure ${lib.optionalString (!withGui) "-nogui"};
+  '';
+
+  buildPhase = ''
+    ./build
+    (cd testing && ../build)
+  '';
+
+  installCheckPhase = "./run_tests";
+  doInstallCheck = true;
+
+  installPhase = ''
+    runHook preInstall
+    mkdir -p $out
+    cp -ar lib $out/lib
+    cp -ar bin $out/bin
+    runHook postInstall
+  '';
+
+  postInstall = ''
+    for prog in $out/bin/*; do
+      wrapProgram $prog --prefix PATH : ${lib.makeBinPath [ ants ]}
+    done
+  '';
+
+  meta = with lib; {
+    homepage = "https://github.com/MRtrix3/mrtrix3";
+    description = "Suite of tools for diffusion imaging";
+    maintainers = with maintainers; [ bcdarwin ];
+    platforms = platforms.linux;
+    license   = licenses.mpl20;
+  };
+}
diff --git a/pkgs/top-level/all-packages.nix b/pkgs/top-level/all-packages.nix
index b002c57c21bd..57870b2a4e76 100644
--- a/pkgs/top-level/all-packages.nix
+++ b/pkgs/top-level/all-packages.nix
@@ -23999,6 +23999,8 @@ in
 
   mrbayes = callPackage ../applications/science/biology/mrbayes { };
 
+  mrtrix = callPackage ../applications/science/biology/mrtrix { python = python3; };
+
   megahit = callPackage ../applications/science/biology/megahit { };
 
   messer-slim = callPackage ../applications/science/biology/messer-slim { };